FLIN4: Fusion of the LIM binding domain of Ldb1 and the N-terminal LIM domain of LMO4
Structural basis for the recognition of ldb1 by the N-terminal LIM domains of LMO2 and LMO4
1H, 15N and 13C assignments of FLIN4, an intramolecular LMO4:ldb1 complex.
Design, production and characterization of FLIN2 and FLIN4: the engineering of intramolecular ldb1:LMO complexes
Fusion protein comprises LIM domain transcription factor LMO4 (residues 16-86) via glycine-rich linker (GGSGGHMGSGG), LIM domain-binding protein 1 (residues 300-339).
fusion of the LIM interacting domain of ldb1 and the N-terminal LIM domain of LMO4
GSLSWKRCAGCGGKIADRFLLYAMDSYWHSRCLKCSSCQAQLGDIGTSSYTKSGMILCRNDYIRLFGNSGAGGSGGHMGS GGDVMVVGEPTLMGGEFGDEDERLITRLENTQFDAANGIDDE
5309 contains Deposition of 1H, 15N and 13C assignments of FLIN4
This structure was solved using triple resonance NMR techniques. In addition to the experiments listed above HNCO, HN(CA)CO, CC(CO)NH-TOCSY and HCC(CO)NH-TOCSY were also used for backbone and side chain assignments.
Structure calculations were performed using ARIA 1.1. Final structures are based on 1804 unambiguous and 81 ambiguous NOE-derived distance restraints and 112 angle constraints from coupling constant data and predictions from chemical shift analysis using TALOS.
simulated annealing molecular dynamics torsion angle dynamics
0.2mM FLIN4, 20mM NaH2PO4, pH7.0, 50mM NaCl, 1mM DTT, 20uM d4-TSP
0.5mM [15N]-FLIN4, 20mM NaH2PO4, pH7.0, 50mM NaCl, 1mM DTT, 20uM d4-TSP
1.0mM [13C,15N]-FLIN4, 20mM NaH2PO4, pH7.0, 50mM NaCl, 1mM DTT, 20uM d4-TSP
LIM domain, fusion protein, LMO proteins, Ldb1, METAL BINDING PROTEIN