ENTHALPIC DESTABILIZATION OF A MUTANT HUMAN LYSOZYME LACKING A DISULFIDE BRIDGE BETWEEN CYSTEINE-77 AND CYSTEINE-95
The Crystal Structure of a Mutant Human Lysozyme C77(Slash)95A with Increased Secretion Efficiency in Yeast
KVFERCELARTLKRLGMDGYRGISLANWMCLAKWESGYNTRATNYNAGDRSTDYGIFQINSRYWCNDGKTPGAVNAAHLS CSALLQDNIADAVACAKRVVRDPQGIRAWVAWRNRCQNRDVRQYVQGCGV
THE ELECTRON DENSITIES OF THE LOOP BETWEEN ALA 73 AND HIS 78 ARE VERY WEAK AND THE ATOMS OF THE LOOP HAVE LARGE B FACTORS. THEN THE LOOP CAN BE VERY FLEXIBLE.
LYSOZYME (E.C.3.2.1.17) MUTANT WITH CYS 77 REPLACED BY ALA (C77A)
MKALIVLGLVLLSVTVQGKVFERCELARTLKRLGMDGYRGISLANWMCLAKWESGYNTRATNYNAGDRSTDYGIFQINSR YWCNDGKTPGAVNACHLSCSALLQDNIADAVACAKRVVRDPQGIRAWVAWRNRCQNRDVRQYVQGCGV