IC3 of CB1 (L431A,A432L) Bound to G(alpha)i
Cannabinoid receptor-G protein interactions: G(alphai1)-bound structures of IC3 and a mutant with altered G protein specificity.
The protein was synthesized using solid phase synthesis. The sequence of the protein is naturally found in Homo sapiens.
transferred NOEs, build up rates from 6 mixing times
back calculated data agree with experimental NOESY spectrum
metric matrix distance geometry to generate initial ensemble of structures; 20 lowest penalty structures used for ensemble-based IRMA refinement
intracellular loop 3 (IC3), cannabinoid 1 receptor (CB1), alpha domain of G protein i, transferred NOEs, SIGNALING PROTEIN