Pseudomonas aeruginosa TolA Domain III, Seleno-methionine Derivative
Structure of the periplasmic domain of Pseudomonas aeruginosa TolA: evidence for an evolutionary relationship with the TonB transporter protein.
(MSE)RALAELLSDTTERQQALADEVGSEVTGSLDDLIVNLVSQQWRRPPSARNG(MSE)SVEVLIE(MSE)LPDGTITN ASVSRSSGDKPFDSSAVAAVRNVGRIPE(MSE)QQLPRATFDSLYRQRRIIFKPEDLSLHHHHHH
MRALAELLSDTTERQQALADEVGSEVTGSLDDLIVNLVSQQWRRPPSARNGMSVEVLIEMLPDGTITNASVSRSSGDKPF DSSAVAAVRNVGRIPEMQQLPRATFDSLYRQRRIIFKPEDLSLHHHHHH
5% wt/v PEG 6000, 100 mM TrisCl, pH 8.0, and 1 mM ZnSO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Domain-Swapping, TolA, TonB, PROTEIN TRANSPORT
RALAELLSDTTERQQALADEVGSEVTGSLDDLIVNLVSQQWRRPPSARNGMSVEVLIEMLPDGTITNASVSRSSGDKPFD SSAVAAVRNVGRIPEMQQLPRATFDSLYRQRRIIFKPEDLSL