DROSOPHILA MELANOGASTER DOUBLESEX (DSX), NMR, 18 STRUCTURES
Sexual dimorphism in diverse metazoans is regulated by a novel class of intertwined zinc fingers.
The Drosophila Doublesex Proteins Share a Novel Zin Finger Related DNA-Binding Domain
Evidence for Evolutionary Conservation of Sex-Determining Genes
The sequence naturally occurs in Drosophila melanogaster (Fruit fly).
NMR EXPERIMENTS WERE CONDUCTED ON VARIAN INOVA 600 MHZ SPECTROMETERS. SOFTWARE VNMR5.1 AND FELIX WERE USED TO PROCESS NMR DATA. STRUCTURE DETERMINATION WAS PERFORMED ON A SILICON GRAPHICS WORK STATION. IN 50 MM DEUTERATED TRIS, 10 MM DEUTERATED DTT.
MOLECULAR MODELS WERE OBTAINED BY DGII IMPLEMENTED IN INSIGHT II (BIOSYM INC., SAN DIEGO, CA), FOLLOWED BY REFINEMENT WITH SIMULATED ANNEALING IN X-PLOR (T.F.HAVEL, A.T.BRUNGER). RESIDUE 1 IN RESTRAINTS CORRESPONDS TO RESIDUE 35 IN DSX. TWO ZINC BINDING SITES ARE: C44,C47,H59,C63 AND H50,C68 C70, C73 EACH IN A TETRAHEDRAL PATTERN. (i.e., 10,13,25,29 and 16,34,36,39 in the coordinates). RESIDUE 35-40 AND 82-105 IN DEX ARE DISORDERED. Structures are giving for residues 35 to 86, corresponding to 1 to 52 numbered in the coordinates. NO MINOR CONFORMERS WERE DETECTED.
DISTANCE GEOMETRY AND SIMULATED ANNEALING
TRANSCRIPTION, DROSOPHILA MELANOGASTER, DNA BINDING, GENE REGULATION
MVSEENWNSDTMSDSDMIDSKNDVCGGASSSSGSSISPRTPPNCARCRNHGLKITLKGHKRYCKFRYCTCEKCRLTADRQ RVMALQTALR