Crystal structure of human matrix metalloproteinase MMP9 (gelatinase B).

Structure of the C-terminally truncated human ProMMP9, a gelatin-binding matrix metalloproteinase.

pro-form with C-terminal domain truncated

APRQRQSTLVLFPGDLRTNLTDRQLAEEYLYRYGYTRVAEMRGESKSLGPALLLLQKQLSLPETGELDSATLKAMRTPRC
GVPDLGRFQTFEGDLKWHHHNITYWIQNYSEDLPRAVIDDAFARAFALWSAVTPLTFTRVYSRDADIVIQFGVAEHGDGY
PFDGKDGLLAHAFPPGPGIQGDAHFDDDELWSLGKGVVVPTRFGNADGAACHFPFIFEGRSYSACTTDGRSDGLPWCSTT
ANYDTDDRFGFCPSERLYTRDGNADGKPCQFPFIFQGQSYSACTTDGRSDGYRWCATTANYDRDKLFGFCPTRADSTVMG
GNSAGELCVFPFTFLGKEYSTCTSEGRGDGRLWCATTSNFDSDKKWGFCPDQGYSLFLVAAHEFGHALGLDHSSVPEALM
YPMYRFTEGPPLHKDDVNGIRHLYG

sodium chloride, Tris, HEPES, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Structure factor analysis for this report was made by SFCHECK V 5.3.4 using anisotropic scaling and the Babinet bulk solvent correction. The correlation between the experimental map and the model was computed using CCP4/SFALL, CCP4/FFT and CCP4/OVERLAPMAP. The difference between the experimental phases and the model phases was computed using CCP4/SFALL and CCP4/PHISTATS.

Twisted beta sheet flanked by helices, HYDROLASE