Solution Structure Of Protein SRP19 Of The Arhaeoglobus fulgidus Signal Recognition Particle, 10 Structures
Solution structure of protein SRP19 of Archaeoglobus fulgidus signal recognition particle.
SIGNAL RECOGNITION PARTICLE 19 KDA PROTEIN
MKESVVWTVNLDSKKSRAEGRRIPRRFAVPNVKLHELVEASKELGLKFRAEEKKYPKSWWEEGGRVVVEKRGTKTKLMIE LARKIAEIREQKREQKKDKKKKKK
Deposition 4935 contains assignments for protein SRP19 of Archaeoglobus fulgidus.
1KVV contains the coordinates for the minimized average structure for the same protein.
IPAP-HSQC experiment was performed in a sample of the protein in either an unstressed or mechanically stressed 8% polyacrylamide gel.
3D 15N-separated NOESY with 13C chemical shift evolution in F2 dimention
The structures are based on a total of 886 restraints, 690 are NOE-derived distance constraints, 130 dihedral angle restraints, 66 1H-15N residual dipolar coupling restraints.
Distance geometry/simulated annealing protocol
25 mM KH2PO4, 50mM NaCl, 95% H2O, 5% D2O
Delaglio, F., Grzesiek, S., Vuister, G., Zhu, W., Pfeifer, J., Bax, A.
Garrett, D.C., Powers, R., Gronenborn, A.M., Clore, G.M.
Brunger, A.T.; Clore, G.M., Gronenborn, A.M., Tjundra, N.
MKECVVWTVNLDSKKSRAEGRRIPRRFAVPNVKLHELVEACKELGLKFRAEEKKYPKSWWEEGGRVVVEKRGTKTKLMIE LARKIAEIREQKREQKKDKKKKKK