Structure of Nitroreductase from E. cloacae Bound with 2e-Reduced Flavin Mononucleotide (FMN)

Structures of nitroreductase in three states: effects of inhibitor binding and reduction.

OXYGEN-INSENSITIVE NAD(P)H NITROREDUCTASE

MDIISVALKRHSTKAFDASKKLTAEEAEKIKTLLQYSPSSTNSQPWHFIVASTEEGKARVAKSAAGTYVFNERKMLDASH
VVVFCAKTAMDDAWLERVVDQEEADGRFNTPEAKAANHKGRTYFADMHRVDLKDDDQWMAKQVYLNVGNFLLGVGAMGLD
AVPIEGFDAAILDEEFGLKEKGFTSLVVVPVGHHSVEDFNATLPKSRLPLSTIVTEC

homopipes, acetate, PEG 4000, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Structure of Nitroreductase from
E. cloacae complex with inhibitor
benzoate

Structure of Nitroreductase from
E. cloacae complex with inhibitor
acetate

Dimer. Chains A and B represent one dimer, chains C and D represent the other dimer; both dimers are in the asymmetric unit. We are depositing all four monomers.

nitroreductase, reduced hydroquinone, flavin, OXIDOREDUCTASE