Crystal structure of phenylalanine-regulated 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (DAHP synthase) from E.coli complexed with Mn2+, PEP, and Phe

Allosteric inhibition of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase alters the coordination of both substrates.

3-deoxy-D-arabino-heptulosonate-7-phosphate synthase

PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE, PHE-SENSITIVE; DAHP synthetase, phenylalanine repressible; PHOSPHO-2-KETO-3-DEOXYHEPTONATE ALDOLASE

(MSE)NYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIHKILKGNDDRLLVVIGPCSIHDPVAAKEYATRL
LALREELKDELEIV(MSE)RVYFEKPRTTVGWKGLINDPH(MSE)DNSFQINDGLRIARKLLLDINDSGLPAAGEFLD
(MSE)ITPQYLADL(MSE)SWGAIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQV(MSE)IDFSHANSSKQFKKQ(MSE)DVCADVCQQIA
GGEKAIIGV(MSE)VESHLVEGNQSLESGEPLAYGKSITDACIGWEDTDALLRQLANAVKARRG

MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIHKILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALR
EELKDELEIVMRVYFEKPRTTVGWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHSAIVNTSGNGDCHIILRGGKEPN
YSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFKKQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAY
GKSITDACIGWEDTDALLRQLANAVKARRG

PEG 4000, lithium sulfate, manganese sulfate, phosphoenolpyruvate, phenylalanine, bis-tris-propane, pH 8.1, VAPOR DIFFUSION, HANGING DROP at 295K

DAHP synthase complexed with Pb2+ and PEP

DAHP synthase complexed with Mn2+ and 2-phosphoglycolate

The biological assembly is a tetramer, two tetramers, ABCD and EFGH, reside in the asymmetric unit.

beta/alpha barrel, allosteric inhibition, feedback regulation, aromatic biosynthetic pathway, LYASE