Crystal Structure of Mouse PITP Alpha Void of Bound Phospholipid at 2.0 Angstroms Resolution

Structure of apo-phosphatidylinositol transfer protein alpha provides insight into membrane association.

Phosphatidylinositol Transfer Protein alpha

VLLKEYRVILPVSVDEYQVGQLYSVAEASKNETGGGEGVEVLVNEPYEKDDGEKGQYTHKIYHLQSKVPTFVRMLAPEGA
LNIHEKAWNAYPYCRTVITNEYMKEDFLIKIETWHKPDLGTQENVHKLEPEAWKHVEAIYIDIADRSQVLSKDYKAEEDP
AKFKSVKTGRGPLGPNWKQELVNQKDCPYMCAYKLVTVKFKWWGLQNKVENFIHKQEKRLFTNFHRQLFCWLDKWVDLTM
DDIRRMEEETKRQLDEMRQKDPVKGMTADD

PET3D CONTAINED PITP cDNA from Swiss mouse 3T3 fibroblast cells

11% PEG 6000, 0.2M calcium acetate, 100MM cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K

1FVZ contains the rat phosphatidylinositol transfer protein alpha with bound phosphatidylcholine

Used maximum likelihood refinement,
and TLS refinement

The biological assembly is a homodimer generated from the monomer in the asymmetric unit by the operation: y, x, -z

PITP, phospholipid binding protein, phospholipid transport, LIPID BINDING PROTEIN