A "three-pronged" binding mechanism for the SAP/SH2D1A SH2 domain: structural basis and relevance to the XLP syndrome.
SIGNALING LYMPHOCYTIC ACTIVATION MOLECULE
MDAVAVYHGKISRETGEKLLLATGLDGSYLLRDSESVPGVYCLCVLYHGYIYTYRVSQTETGSWSAETAPGVHKRYFRKI KNLISAFQKPDQGIVIPLQYPVEKKSSARSTQGTTGIREDPDVCLKAP
This peptide was chemically synthesized (solid phase synthesis). The sequence of the peptide is naturally found in Homo sapiens (human).
The structure was determined using triple-resonance NMR spectroscopy.
1 mM SAP U-15N, 13C; 1mM n-pY; 20 mM phosphate buffer, 100mM NaCl
SH2 domain, protein-peptide complex, IMMUNE SYSTEM