CRYSTAL STRUCTURE OF HUMAN TYROSYL-DNA PHOSPHODIESTERASE (TDP1)

The crystal structure of human tyrosyl-DNA phosphodiesterase, Tdp1.

LEDPGEGQDIWD(MSE)LDKGNPFQFYLTRVSGVKPKYNSGALHIKDILSPLFGTLVSSAQFNYCFDVDWLVKQYPPEFR
KKPILLVHGDKREAKAHLHAQAKPYENISLCQAKLDIAFGTHHTK(MSE)(MSE)LLLYEEGLRVVIHTSNLIHADWHQK
TQGIWLSPLYPRIADGTHKSGESPTHFKANLISYLTAYNAPSLKEWIDVIHKHDLSETNVYLIGSTPGRFQGSQKDNWGH
FRLKKLLKDHASS(MSE)PNAESWPVVGQFSSVGSLGADESKWLCSEFKES(MSE)LTLGKESKTPGKSSVPLYLIYPSV
ENVRTSLEGYPAGGSLPYSIQTAEKQNWLHSYFHKWSAETSGRSNA(MSE)PHIKTY(MSE)RPSPDFSKIAWFLVTSAN
LSKAAWGALEKNGTQL(MSE)IRSYELGVLFLPSALGLDSFKVKQKFFAGSQEP(MSE)ATFPVPYDLPPELYGSKDRPW
IWNIPYVKAPDTHGN(MSE)WVPS

LEDPGEGQDIWDMLDKGNPFQFYLTRVSGVKPKYNSGALHIKDILSPLFGTLVSSAQFNYCFDVDWLVKQYPPEFRKKPI
LLVHGDKREAKAHLHAQAKPYENISLCQAKLDIAFGTHHTKMMLLLYEEGLRVVIHTSNLIHADWHQKTQGIWLSPLYPR
IADGTHKSGESPTHFKANLISYLTAYNAPSLKEWIDVIHKHDLSETNVYLIGSTPGRFQGSQKDNWGHFRLKKLLKDHAS
SMPNAESWPVVGQFSSVGSLGADESKWLCSEFKESMLTLGKESKTPGKSSVPLYLIYPSVENVRTSLEGYPAGGSLPYSI
QTAEKQNWLHSYFHKWSAETSGRSNAMPHIKTYMRPSPDFSKIAWFLVTSANLSKAAWGALEKNGTQLMIRSYELGVLFL
PSALGLDSFKVKQKFFAGSQEPMATFPVPYDLPPELYGSKDRPWIWNIPYVKAPDTHGNMWVPS

PEG 8000, sodium chloride, CHES, spermine, pH 9.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K

GEGQDIWDMLDKGNPFQFYLTRVSGVKPKYNSGALHIKDILSPLFGTLVSSAQFNYCFDVDWLVKQYPPEFRKKPILLVH
GDKREAKAHLHAQAKPYENISLCQAKLDIAFGTHHTKMMLLLYEEGLRVVIHTSNLIHADWHQKTQGIWLSPLYPRIADG
THKSGESPTHFKADLISYLMAYNAPSLKEWIDVIHKHDLSETNVYLIGSTPGRFQGSQKDNWGHFRLKKLLKDHASSMPN
AESWPVVGQFSSVGSLGADESKWLCSEFKESMLTLGKESKTPGKSSVPLYLIYPSVENVRTSLEGYPAGGSLPYSIQTAE
KQNWLHSYFHKWSAETSGRSNAMPHIKTYMRPSPDFSKIAWFLVTSANLSKAAWGALEKNGTQLMIRSYELGVLFLPSAF
GLDSFKVKQKFFAGSQEPMATFPVPYDLPPELYGSKDRPWIWNIPYVKAPDTHGNMWVPS