Crystal structure of Sulfolobus solfataricus elongation factor 1 alpha in complex with GDP

The crystal structure of Sulfolobus solfataricus elongation factor 1alpha in complex with GDP reveals novel features in nucleotide binding and exchange.

Crystallization of a hyperthermophilic archeal elongation factor 1 alpha

EF-1-ALPHA; ELONGATION FACTOR TU; EF-TU; tuF-1

MSQKPHLNLIVIGHVDHGKSTLVGRLLMDRGFIDEKTVKEAEEAAKKLGKESEKFAFLLDRLKEERERGVTINLTFMRFE
TKKYFFTIIDAPGHRDFVKNMITGASQADAAILVVSAKKGEYEAGMSVEGQTREHIILAKTMGLDQLIVAVNKMDLTEPP
YDEKRYKEIVDQVSKFMRSYGFNTNKVRFVPVVAPSGDNITHKSENMKWYNGPTLEEYLDQLELPPKPVDKPLRIPIQDV
YSISGVGTVPVGRVESGVLKVGDKIVFMPAGKVGEVRSIETHHTKMDKAEPGDNIGFNVRGVEKKDIKRGDVVGHPNNPP
TVADEFTARIIVVWHPTALANGYTPVLHVHTASVACRVSELVSKLDPRTGQEAEKNPQFLKQGDVAIVKFKPIKPLCVEK
YNEFPPLGRFAMRDMGKTVGVGIIVDVKPAKVEIK

PEG 4000, magnesium chloride, propan-2-ol, citrate buffer, pH 5.6, MICROBATCH UNDER OIL at 277K

GTPASE, ALPHA/BETA STRUCTURE, PROTEIN BIOSYNTHESIS, TRANSLATION

MSQKPHLNLIVIGHIDHGKSTLVGRLLMDRGFIDEKTVKEAEEAAKKLGKESEKFAFLLDRLKEERERGVTINLTFMRFE
TKKYFFTIIDAPGHRDFVKNMITGASQADAAILVVSAKKGEYEAGMSVEGQTREHIILAKTMGLDQLIVAVNKMDLTEPP
YDEKRYKEIVDQVSKFMRSYGFNTNKVRFVPVVAPAGDNITHRSENMKWYNGPTLEEYLDQLELPPKPVDKPLRIPIQDV
YSISGVGTVPVGRVESGVLKVGDKIVFMPAGKVGEVRSIETHHTKMDKAEPGDNIGFNVRGVEKKDIKRGDVVGHPNNPP
TVADEFTARIIVVWHPTALANGYTPVIHVHTASVACRVSELVSKLDPRTGQEAEKNPQFLKQGDVAIVKFKPIKPLCVEK
YNEFPPLGRFAMRDMGKTVGVGIIVDVKPAKVEIK