NMR Structure of the E. coli Peptidyl-Prolyl cis/trans-Isomerase Parvulin 10
Solution structure of Escherichia coli Par10: The prototypic member of the Parvulin family of peptidyl-prolyl cis/trans isomerases.
AKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSGKRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQ FGYHIIKVLYRN
The structure was determined using triple-resonance NMR spectroscopy.
the structures are based on 1097 NOE-derived distance constraints, 68 dihedral restraints for CSI-derived helical regions, 42 3J(HN,HA) restraints, 30 distance constraints for hydrogen bonds based on CSI and MEXICO data.
The averaged structure of the ensemble (1JNS) was regularized under experimental constraints
0.8 mM [U-15N] Parvulin 10, 10 mM phosphate buffer, pH 6.0, 100 mM KCl, 1 mM EDTA, 1 mM DTE
0.8 mM [U-13C, 15N] Parvulin 10, 10 mM phosphate buffer, pH 6.0, 100 mM KCl, 1 mM EDTA, 1 mM DTE
ALPHA-BETA SANDWICH, CIS PEPTIDE BOND, ISOMERASE