HUMAN INTERLEUKIN 3 (IL-3) MUTANT WITH TRUNCATION AT BOTH N-AND C-TERMINI AND 14 RESIDUE CHANGES, NMR, MINIMIZED AVERAGE STRUCTURE
Three-dimensional solution structure and backbone dynamics of a variant of human interleukin-3.
1H, 13C, and 15N NMR Resonance Assignments, Secondary Structure, and Backbone Topology of a Variant of Human Interleukin-3
ANCSIMIDEIIHHLKRPPNPLLDPNNLNSEDMDILMERNLRTPNLLAFVRAVKHLENASAIESILKNLLPCLPLATAAPT RHPIHIKDGDWNEFRRKLTFYLKTLENAQAQQ
AS IN X-PLOR PARALLHDG.PRO STRUCTURES WERE CALCULATED USING A SIMULATED ANNEALING PROTOCOL IN X-PLOR. INPUT CONSTRAINTS ARE AS FOLLOWS: 1659 NUCLEAR OVERHAUSER ENHANCEMENT (NOE) (799 INTRA-RESIDUE; 342 SEQUENTIAL (I-J =1); 236 MEDIUM-RANGE INTERRESIDUE (<1 I-J <=5); 282 LONG-RANGE INTERRESIDUE (I-J >5)); 38 PAIRS OF HYDROGEN-BOND RESTRAINTS; 76 PHI TORSION ANGLE RESTRAINTS. PSEUDOATOM POSITIONS WERE USED FOR CONSTRAINTS INVOLVING METHYLENE, AROMATIC, AND METHYL PROTONS. THE COORDINATES DEPOSITED HERE ARE OBTAINED BY AVERAGING 25 CONVERGED STRUCTURES PRIOR TO A RESTRAINED ENERGY MINIMIZATION. A COMPARISON OF THE FAMILY OF THE 25 STRUCTURES WITH THE AVERAGED STRUCTURE GIVES RMSD VALUES OF 0.88 ANGSTROMS FOR ALL (N,CA,C') EXCEPT RESIDUES 28 - 39, AND 0.41 ANGSTROMS FOR (N,CA,C') OF HELICAL REGIONS (RESIDUES 16 - 26, 42 - 49, 54 - 67, 72 - 84, AND 104 - 122).
HEMATOPOIETIC GROWTH FACTOR, COLONY-STIMULATING FACTOR, CYTOKINE
MSRLPVLLLLQLLVRPGLQAPMTQTTPLKTSWVNCSNMIDEIITHLKQPPLPLLDFNNLNGEDQDILMENNLRRPNLEAF NRAVKSLQNASAIESILKNLLPCLPLATAAPTRHPIHIKDGDWNEFRRKLTFYLKTLENAQAQQTTLSLAIF