Holo 1L-myo-inositol-1-phosphate Synthase

The crystal structure and mechanism of 1-L-myo-inositol- 1-phosphate synthase

MTEDNIAPITSVKVVTDKCTYKDNELLTKYSYENAVVTKTASGRFDVTPTVQDYVFKLDLKKPEKLGIMLIGLGGNNGST
LVASVLANKHNVEFQTKEGVKQPNYFGSMTQCSTLKLGIDAEGNDVYAPFNSLLPMVSPNDFVVSGWDINNADLYEAMQR
SQVLEYDLQQRLKAKMSLVKPLPSIYYPDFIAANQDERANNCINLDEKGNVTTRGKWTHLQRIRRDIQNFKEENALDKVI
VLWTANTERYVEVSPGVNDTMENLLQSIKNDHEEIAPSTIFAAASILEGVPYINGSPQNTFVPGLVQLAEHEGTFIAGDD
LKSGQTKLKSVLAQFLVDAGIKPVSIASYNHLGNNDGYNLSAPKQFRSKEISKSSVIDDIIASNDILYNDKLGKKVDHCI
VIKYMKPVGDSKVAMDEYYSELMLGGHNRISIHNVCEDSLLATPLIIDLLVMTEFCTRVSYKKVDPVKEDAGKFENFYPV
LTFLSYWLKAPLTRPGFHPVNGLNKQRTALENFLRLLIGLPSQNELRFEERLL

PEG 8000, sodium acetate, water, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

myo-Inositol-1-phosphate synthase complexed with an inhibitor, 2-deoxy-glucitol-6-phosphate

myo-inositol-1-phosphate synthase (E.C.5.5.1.4)

The biological assembly is a tetramer. The tetramer has 222 symmetry with a non-crystallographic 2-fold axis relating the dimer in the asymmetric unit and a crystallographic 2-fold axis relating the two dimers that make up the tetramer. The assymmetric unit operations are x, y, z and -x, y, -z.