NMR SOLUTION STRUCTURE OF AN EF-HAND CALCIUM BINDING PROTEIN FROM ENTAMOEBA HISTOLYTICA
NMR derived solution structure of an EF-hand calcium-binding protein from Entamoeba Histolytica.
MAEALFKEIDVNGDGAVSYEEVKAFVSKKRAIKNEQLLQLIFKSIDADGNGEIDQNEFAKFYGSIQGQDLSDDKIGLKVL YKLMDVDGDGKLTKEEVTSFFKKHGIEKVAEQVMKADANGDGYITLEEFLEFSL
1JFK contains same protein complex with calcium.
4271 contains assigned chemical shift lists
The structure was determined using triple-resonance NMR spectroscopy
structures with the least restraint violations
The structure is based on a total of 1265 NOE-derived distance constraints, 200 dihedral angle constraints
3mM EHCaBP, 30mM Deuterated Tris buffer, 30mM Calc2
3mM EHCaBP, 30mM Deuterated Tris buffer, 30mM Cacl2
EF-HAND, HELIX-LOOP-HELIX, CALCIUM-BINDING PROTEIN, METAL BINDING PROTEIN