Solution Structure of TM006 Protein from Thermotoga maritima
An NMR approach to structural proteomics.
GSSHHHHHHSSGLVPRGSHMAKYQVTKTLDVRGEVCPVPDVETKRALQNMKPGEILEVWIDYPMSKERIPETVKKLGHEV LEIEEVGPSEWKIYIKVK
Structure was determined using 2D and 3D experiments for unlabelled, 15N-labelled and double-labelled protein.
After determination of protein fold by using manual NOE assigments, values of coupling constants and 13C chemical shifts, automatic peak NOE assigments were made by using ARIA and the structure refined by using standard protocol in CNS.
2 mM TM006 protein U-15N,13C; 50mM phosphate buffer
2 mM TM006 protein U-15N; 50mM phosphate buffer
5 mM TM006 unlabelled protein; 50mM phosphate buffer
TM006, Thermotoga maritima, STRUCTURAL GENOMICS