Solution structure of the monomeric [Thr(B27)->Pro,Pro(B28)->Thr] insulin mutant (PT insulin)
Flexibility and bioactivity of insulin: an NMR investigation of the solution structure and folding of an unusually flexible human insulin mutant with increased biological activity.
197 NOEs, 52 dihedral angles, 11 hydrogen bonds. Force constants: k(NOE)= 50 kcal mol-1 AA-1, k(DIHE)= 200 kcal mol-1 AA-1
2.8 mM PT-insulin; 35% Trifluoroethanol; 5% D2O; 60% H2O
2.8 mM PT-insulin; 35% Trifluoroethanol; 65% D2O;
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer, Bax
Bartels, Xia, Billeter, Guntert, Wuthrich
Helix-turn-helix, Coil-helix-coil, HORMONE-GROWTH FACTOR COMPLEX