The Crystal Structure of Haemophilus Influenzae HI1715, an Oligoribonuclease
SH(MSE)SFDKQNLIWIDLE(MSE)TGLDPEKERIIEIATIVTDKNLNILAEGPVLAVHQSDELLNK(MSE)NDWCQKTH SENGLIERIKASKLTERAAELQTLDFLKKWVPKGASPICGNSIAQDKRFLVKY(MSE)PDLADYFHYRHLDVSTLKELAA RWKPEILEGFKKENTHLALDDIRESIKELAYYREHF(MSE)KLD
SHMSFDKQNLIWIDLEMTGLDPEKERIIEIATIVTDKNLNILAEGPVLAVHQSDELLNKMNDWCQKTHSENGLIERIKAS KLTERAAELQTLDFLKKWVPKGASPICGNSIAQDKRFLVKYMPDLADYFHYRHLDVSTLKELAARWKPEILEGFKKENTH LALDDIRESIKELAYYREHFMKLD
Protein solution: 15 mg/mL protein in 0.01 M HEPES, 0.15 M sodium chloride, 0.005 M DTT, 0.005 M EDTA with pH 7.1-8.0. Reservoir: 2.3 M ammonium sulfate at pH 7.1-7.5. Hanging drop vapor diffusion method. Drops were formed by combining 4 microliters reservoir solution with 4 microliters protein solution. Room temperature., pH 7.3
ANISOTROPIC SCALING APPLIED BY THE METHOD OF PARKIN, MOEZZI & HOPE, J.APPL.CRYST.28(1995)53-56
MOEWS & KRETSINGER, J.MOL.BIOL.91(1973)201-228
RIBONUCLEASE, EXORIBONUCLEASE, OLIGORIBONUCLEASE, HAEMOPHILUS INFLUENZAE, Structure 2 Function Project, S2F, Structural Genomics, HYDROLASE
MSFDKQNLIWIDLEMTGLDPEKERIIEIATIVTDKNLNILAEGPVLAVHQSDELLNKMNDWCQKTHSENGLIERIKASKL TERAAELQTLDFLKKWVPKGASPICGNSIAQDKRFLVKYMPDLADYFHYRHLDVSTLKELAARWKPEILEGFKKENTHLA LDDIRESIKELAYYREHFMKLD