Solution Structure of the Unmodified Anticodon Stem-loop from E. coli tRNA(Phe)
Solution conformations of unmodified and A(37)N(6)-dimethylallyl modified anticodon stem-loops of Escherichia coli tRNA(Phe).
T7 RNA polymerase in vitro transcription
The structure was determined using 1H, 13C, 15N and 31P heteronuclear NMR spectroscopy.
294 NOE distance constraints, 36 base pair constraints, 70 torsion angle constraints. Global fold without torsion angle constraints. Refinement with torsion angle constraints.
10 mM Potassium Phosphate, 10mM NaCl, 0.02mM EDTA. 90% H2O-10% D2O or 100% D2O.
RNA stem-loop, trinucleotide loop, hairpin, RNA