Crystal structure of HyHEL-10 Fv mutant LS91A complexed with hen egg white lysozyme

The Role of Hydrogen Bonding via Interfacial Water Molecules in Antigen-Antibody Complexation. THE HyHEL-10-HEL INTERACTION

lysozyme binding Ig kappa chain V23-J2 region

DIVLTQSPATLSVTPGNSVSLSCRASQSIGNNLHWYQQKSHESPRLLIKYASQSISGIPSRFSGSGSGTDFTLSINSVET
EDFGMYFCQQANSWPYTFGGGTKLEIK

DVQLQESGPSLVKPSQTLSLTCSVTGDSITSDYWSWIRKFPGNRLEYMGYVSYSGSTYYNPSLKSRISITRDTSKNQYYL
DLNSVTTEDTATYYCANWDGDYWGQGTLVTVSAA

KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNTDGSTDYGILQINSRWWCNDGRTPGSRNLCNIPC
SALLSSDITASVNCAKKIVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL

PEG 6000, Hepes, glycerol, methyl-pentanediol, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K

HyHEL-10 Fv Wild Type complexed with hen egg white lysozyme

HyHEL-10 Fv mutant LY50F complexed with hen egg white lysozyme

HyHEL-10 Fv mutant LS93A complexed with hen egg white lysozyme

ANTIGEN-ANTIBODY COMPLEX, IMMUNE SYSTEM-HYDROLASE COMPLEX

DIVLTQSPATLSVTPGNSVSLSCRASQSIGNNLHWYQQKSHESPRLLIKYASQSISGIPSRFSGSGSGTDFTLSINSVET
EDFGMYFCQQSNSWPYTFGGGTKLEIK

DVQLQESGPSLVKPSQTLSLTCSVTGDSITSDYWSWIRKFPGNRLEYMGYVSYSGSTYYNPSLKSRISITRDTSKNQYYL
DLNSVTTEDTATYYCANWDGDYWGQGTLVTVSA