Pyrococcus furiosus PCNA mutant (Met73Leu, Asp143Ala, Asp147Ala): orthorhombic form

Intermolecular ion pairs maintain the toroidal structure of Pyrococcus furiosus PCNA

MPFEIVFEGAKEFAQLIDTASKLIDEAAFKVTEDGISMRAMDPSRVVLIDLNLPSSIFSKYEVVEPETIGVNLDHLKKIL
KRGKAKDTLILKKGEENFLEITIQGTATRTFRVPLIDVEEMEVDLPELPFTAKVVVLGEVLKAAVKAASLVSDSIKFIAR
ENEFIMKAEGETQEVEIKLTLEDEGLLDIEVQEETKSAYGVSYLSDMVKGLGKADEVTIKFGNEMPMQMEYYIRDEGRLT
FLLAPRVEE

ammonium sulfate, 2-methyl-2,4-pentanediol, sodium citrate, glycerol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

1GE8 contains the Met73Leu mutant of the same protein.

1IZ4 contains the Met73Leu, Asp143Ala mutant of the same protein, tetragonal form

DNA, REPLICATION, PROCESSIVITY, SLIDING CLAMP, DNA binding protein

MPFEIVFEGAKEFAQLIDTASKLIDEAAFKVTEDGISMRAMDPSRVVLIDLNLPSSIFSKYEVVEPETIGVNMDHLKKIL
KRGKAKDTLILKKGEENFLEITIQGTATRTFRVPLIDVEEMEVDLPELPFTAKVVVLGEVLKDAVKDASLVSDSIKFIAR
ENEFIMKAEGETQEVEIKLTLEDEGLLDIEVQEETKSAYGVSYLSDMVKGLGKADEVTIKFGNEMPMQMEYYIRDEGRLT
FLLAPRVEE