alpha-L-fucose; 6-deoxy-alpha-L-galactopyranose; L-fucose; fucose

beta-L-fucose; 6-deoxy-beta-L-galactopyranose; L-fucose; fucose; 6-DEOXY-BETA-L-GALACTOSE

beta-D-galactose; D-galactose; galactose

N-acetyl-beta-D-glucosamine; 2-acetamido-2-deoxy-beta-D-glucose; 2-acetamido-2-deoxy-D-glucose; 2-acetamido-2-deoxy-glucose; N-ACETYL-D-GLUCOSAMINE

Refined structure of an intact IgG2a monoclonal antibody.

The Three-Dimensional Structure of an Intact Monoclonal Antibody for Canine Lymphoma

SUPPER GRAPHITE CRYSTAL ASYMMETRIC CUT TRIANGULAR BENT CRYSTAL (SI-111)

DIVLTQSPSSLSASLGDTITITCHASQNINVWLSWYQQKPGNIPKLLIYKASNLHTGVPSRFSGSGSGTGFTLTISSLQP
EDIATYYCQQGQSYPLTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVL
NSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC

EVKLQESGGGLVQPGGSLKLSCATSGFTFSDYYMYWVRQTPEKRLEWVAYISNGGGSTYYPDTVKGRFTISRDNAKNTLY
LQMSRLKSEDTAMYYCARHGGYYAMDYWGQGTTVTVSSAKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWN
SGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPRGPTIKPCPPCKCPAPNLLGGPS
VFIFPPKIKDVLMISLSPIVTCVVVDVSEDDPDVQISWFVNNVEVHTAQTQTHREDYNSTLRVVSALPIQHQDWMSGKEF
KCKVNNKDLPAPIERTISKPKGSVRAPQVYVLPPPEEEMTKKQVTLTCMVTDFMPEDIYVEWTNNGKTELNYKNTEPVLD
SDGSYFMYSKLRVEKKNWVERNSYSCSVVHEGLHNHHTTKSFSR

THE MULTIWIRE AND SYNCHROTRON DATA SETS WERE CUT AT F > 4SIGMA, AND THEN MERGED TO YIELD THE FINAL REDUCED DATA SET OF 41371 UNIQUE REFLECTIONS AT 2.8-20 A; RMERGE = 6.7%. THIS COMBINED DATA WAS USED FOR REFINEMENT.

7 MICROLITERS OF 4.3 MG/ML MAB231, 3 MICROLITERS 50 MILLIMOLAR TRIS PH 8.0, AND 7 MICROLITERS 4% PEG 3350 EQUILIBRATED AGAINST 25 ML RESERVOIRS OF 4% PEG 3350, AT 18 DEGREE CELSIUS (SANDWICH PLATE)., temperature 291K

THE INTACT ANTIBODY IS NUMBERED ACCORDING TO THE CONVENTION OF E. KABAT [KABAT ET AL. (1991) SEQUENCES OF PROTEINS OF IMMUNOLOGICAL INTEREST, 5TH ED., NATIONAL INSTITUTES OF HEALTH, BETHESDA, MD].

VL:VH DOMAIN PAIR OF PDB ENTRY 1MCP, CL:CH1 DOMAIN PAIR OF ENTRY 2HFL, FC FRAGMENT OF ENTRY 1FC1

BULK SOLVENT MODEL USED GROUP 1 POSITIONAL (CL) (A) : 0.05 ; 200 GROUP 2 POSITIONAL (CH1) (A) : 0.05 ; 200 GROUP 3 POSITIONAL (CH2) (A) : 0.06 ; 200 GROUP 4 POSITIONAL (CH3) (A) : 0.05 ; 200 BACKBONE ATOMS ONLY WERE RESTRAINED BY NCS AND RESIDUES INVOLVED IN PACKING WERE NOT RESTRAINED. NCS B-FACTOR RESTRAINTS WERE NOT EMPLOYED.

THERE IS ONE ENTIRE ANTIBODY MOLECULE PER ASYMMETRIC UNIT.

INTACT IMMUNOGLOBULIN V REGION C REGION, IMMUNOGLOBULIN

AKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSI
TCNVAHPASSTKVDKKIEPRGPTIKPCPPCKCPAPNLLGGPSVFIFPPKIKDVLMISLSPIVTCVVVDVSEDDPDVQISW
FVNNVEVHTAQTQTHREDYNSTLRVVSALPIQHQDWMSGKEFKCKVNNKDLPAPIERTISKPKGSVRAPQVYVLPPPEEE
MTKKQVTLTCMVTDFMPEDIYVEWTNNGKTELNYKNTEPVLDSDGSYFMYSKLRVEKKNWVERNSYSCSVVHEGLHNHHT
TKSFSRTPGK