CRYSTAL STRUCTURE OF NH3-DEPENDENT NAD+ SYNTHETASE FROM BACILLUS SUBTILIS COMPLEXED WITH TWO MOLECULES DEAMIDO-NAD

Stabilization of active-site loops in NH3-dependent NAD+ synthetase from Bacillus subtilis.

SMQEKIMRELHVKPSIDPKQEIEDRVNFLKQYVKKTGAKGFVLGISGGQDSTLAGRLAQLAVESIREEGGDAQFIAVRLP
HGTQQDEDDAQLALKFIKPDKSWKFDIKSTVSAFSDQYQQETGDQLTDFNKGNVKARTRMIAQYAIGGQEGLLVLGTDHA
AEAVTGFFTKYGDGGADLLPLTGLTKRQGRTLLKELGAPERLYLKEPTADLLDEKPQQSDETELGISYDEIDDYLEGKEV
SAKVSEALEKRYSMTEHKRQVPASMFDDWWK

sodium acetate buffer, Magnesium chloride, PEG 400, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K

1ee1 contains the same protein compexed with one molecule ATP, two molecules Deamido-NAD and one MG2+ ion

1fyd contains the same protein complexed with one molecule AMP, one pyrophosphate ion and one MG2+ ion

The second part of the biological assembly is generated by the two fold axis

LYASE, AMIDOTRANSFERASE, NH3 DEPENDENT, ATP, PYROPHOSPHATASE, LIGASE