SOLUTION STRUCTURE AND FUNCTION OF A CONSERVED PROTEIN SP14.3 ENCODED BY AN ESSENTIAL STREPTOCOCCUS PNEUMONIAE GENE
GSGVDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVDKPEGITLNDTADLTEMISPVLDTIKPDPFPEQY FLEITSPGLERPLKTKDAVAGAVGKYIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAV KLLE
The structure was determined using triple-resonance NMR spectroscopy.
distance geometry/simulated annealing protocol
1 mM SP14.3 15N-labeled, 15N,13C-labeled, and 15N,13C,2H-labeled with selectively protonated methyl groups. 50 mM BisTris HCl, pH 6.5, 50 mM ammonium sulphate.
NUCLEIC ACID BINDING PROTEIN, RIBOSOMAL PROTEIN, ESSENTIAL GENE, STRUCTURAL GENOMICS