NMR STRUCTURE OF THE UBX DOMAIN FROM P47
Solution structure and interaction surface of the C-terminal domain from p47: a major p97-cofactor involved in SNARE disassembly.
KASSSILINEAEPTTNIQIRLADGGRLVQKFNHSHRISDIRLFIVDARPAMAATSFVLMTTFPNKELADENQTLKEANLL NAVIVQRLT
back calculated data agree with experimental NOESY spectrum,structures with acceptable covalent geometry,structures with favorable non-bond energy,structures with the least restraint violations,structures with the lowest energy
HNCA, HN(CO)CA, CBCA(CO)NH, HBHA(CO)NH, 15N-NOESY
1mM 13C, 15N-labelled p47 C domain sample
20mM NaAc buffer pH5.2; 90% H2O, 10% D2O
Ubiquitin superfold, UBX, unusual N-terminal feature, PROTEIN BINDING