CRYSTAL STRUCTURE OF ESCHELICHIA COLI BRANCHED-CHAIN AMINO ACID AMINOTRANSFERASE.

Structures of Escherichia coli branched-chain amino acid aminotransferase and its complexes with 4-methylvalerate and 2-methylleucine: induced fit and substrate recognition of the enzyme.

BRANCHED-CHAIN AMINO ACID AMINOTRANSFERASE

MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYDSHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELM
EACRDVIRKNNLTSAYIRPLIFVGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSALPGITRDAIIKLAKELGIEVREQ
VLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEGRCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ

PEG400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

1I1L contains the same protein complexed with 2-methylleucine

1I1M contains the same protein complexed with 4-methylvarelate

AMINOTRANSFERASE(E.C.2.6.1.42), PYRIDOXAL-5'-PHOSPHATE

AMINOTRANSFERASE, HEXAMER, PLP, TRANSFERASE