RTD-1, A CYCLIC ANTIMICROBIAL DEFENSIN FROM RHESUS MACAQUE LEUKOCYTES
Three-dimensional structure of RTD-1, a cyclic antimicrobial defensin from Rhesus macaque leukocytes.
SEQUENCE RESIDUES NUMBERED ACCORDING TO TANG ET AL., SCIENCE 286 (1999), PP. 498-502
The peptide was chemically synthesized. This sequence occurs naturally in rhesus macaques (mucaca mulatta)
This structure was determined using standard 2D homonuclear techniques.
structures with the least restraint violations,structures with the lowest energy
The structures are based on a total of 94 restraints, 87 are NOE-derived distance constraints, 7 dihedral angle restraints
torsion angle dynamics, simulated annealing, explicit inclusion of water molecules
1mM RTD-1, 20mM sodium phosphate pH 4.5, 80% H2O, 10% D2O, 10% acetonitrile-d3
1mM RTD-1, 20mM sodium phosphate pH 4.5, 90% H2O, 10% D2O
1mM RTD-1, 20mM sodium phosphate pH 4.5, 99% D2O
cyclic, beta-sheet, disulfide-rich, ANTIMICROBIAL PROTEIN