REFINED STRUCTURES AT 2 ANGSTROMS AND 2.2 ANGSTROMS OF THE TWO FORMS OF THE H-PROTEIN, A LIPOAMIDE-CONTAINING PROTEIN OF THE GLYCINE DECARBOXYLASE COMPLEX
6-(HYDROXYETHYLDITHIO)-8-(AMINOMETHYLTHIO)OCTANOIC ACID
The lipoamide arm in the glycine decarboxylase complex is not freely swinging.
X-Ray Structure Determination at 2.6 Angstroms Resolution of a Lipoate-Containing Protein: The H-Protein of the Glycine Decarboxylase Complex from Pea Leaves
SNVLDGLKYAPSHEWVKHEGSVATIGITDHAQDHLGEVVFVELPEPGVSVTKGKGFGAVESVKATSDVNSPISGEVIEVN TGLTGKPGLINSSPYEDGWMIKIKPTSPDELESLLGAKEYTKFCEEEDAAH
COMPND H-PROTEIN CHARGED IN METHYLAMINE OF THE GLYCINE DECARBOXYLASE COMPLEX FROM PEA THE H-PROTEIN PROVIDES THE ATTACHMENT SITE FOR THE LIPOIC ACID VIA AN AMIDE LINKAGE TO THE EPSILON-AMINO GROUP OF A LYS63.
SEQUENCE REFERENCE DATABASE: SWISS-PROT ENTRY_NAME: GCSH_PEA REFERENCE: MACHEREL D., LEBRUN M.,GAGNON J., NEUBURGER M., DOUCE R. BIOCHEM J. 268:783-789(1990) KIM Y., OLIVER D.J J. BIOL. CHEM. 265: 848-853(1990).
H-PROTEIN (E.C.1.4.4.2) COMPLEXED WITH LIPOIC ACID CHARGED IN METHYLAMINE
MALRMWASSTANALKLSSSSRLHLSPTFSISRCFSNVLDGLKYAPSHEWVKHEGSVATIGITDHAQDHLGEVVFVELPEP GVSVTKGKGFGAVESVKATSDVNSPISGEVIEVNTGLTGKPGLINSSPYEDGWMIKIKPTSPDELESLLGAKEYTKFCEE EDAAH