Nucleotide-Dependent Conformational Changes in a Protease-Associated ATPase HslU

Nucleotide-dependent conformational changes in a protease-associated ATPase HsIU.

Mutational Studies of Hslu and its Docking Mode With Hslv.

The Structures of Hslu and the ATP-Dependent Protease HslU-HslV.

Crystal and Solution Structures of an HslUV Protease-chaperone Complex.

Crystal Structures of the Hslvu Peptidase-ATPase Complex Reveal an ATP-Dependent Proteolysis Mechanism

ATP-dependent proteases: Docking of Components in a Bacterial Complex

A corrected quaternary arrangement of the peptidase hslv and atpase hslu in a cocrystal structure

ATP-DEPENDENT HSL PROTEASE ATP-BINDING SUBUNIT HSLU

TTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVIAGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAK
DWRTDRMLRKLEALLAVADETASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDICI
YTNHFHTIEELSYKA

HHHHHHHSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEELRHEVTPKNILMIGPTGVGKTEIARRLAKLA
NAPFIKVEATKFTEVGYVGKEVDSIIRDLTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSA
ARQAFRKKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKIKDAMKLLIEEEAAKLV
NPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREGVQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAK
PSDLIPELQGRLPIRVELQALTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGARR
LHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL

THIS ENTRY CONTAINS A SOLUTION IN X-RAY STRUCTURES TO THE X-RAY DIFFRACTION DATA THAT WERE RETRIEVED FROM PDB DATABASE UNDER ACCESSION NUMBER 1E94. THIS ENTRY IS RELATED TO 1HQY, 1HT2 AND 1E94.

THE ASSIGNMENT OF THE SCREW AXIS 6(3) IN THE P6(3)22 SPACE GROUP FOR THE HSLVU COMPLEX STRUCTURES DESCRIBED IN REFERENCES 1 AND 2 (CORRESPONDING PDB ACCESSION NUMBERS ARE 1DOO AND 1E94, RESPECTIVELY) REQUIRES THE PRESENCE OF SYSTEMATIC EXTINCTIONS ALONG (00L) WITH L=2N+1. THERE WERE NO SYSTEMATIC EXTINCTIONS AT ALL IN THE 1E94SF ENTRY. THERE WERE TWO REFLECTIONS WITH F/SIGMA(F) NEAR 20 AND NINE REFLECTIONS WITH F/SIMGA(F) OVER 10 ALONG (00L) WITH L=2N+1. SUCH A LARGE NUMBER OF SIGNIFICANT OBSERVATIONS CANNOT BE DUE TO TECHNICAL ERRORS IN MEASUREMENT OF X-RAY DIFFRACTION DATA. A STATISTICAL ANALYSIS OF THEM COMPARED WITH THE REST OF THE DATA CONFIRMS THAT THEY ARE NOT DUE TO TECHNICAL ERRORS. THEREFORE, THE SPACE GROUP MUST NOT BE P6(3)22.

LARGE VALUES OF COMBINED R-MERGE VALUES FOR OBSERVED DATA 1DOO (14.1%) AND 1E94 (12.1%) ARE INDICATIVE OF INCORRECT ASSIGNEMENT OF POINT SYMMETRY GROUP TO BE 622. THE ESTIMATED MEASUREMENT PRECISION IN INTENSITY SHOULD BE ABOUT 1% ON THE BASIS OF THE AVERAGE F/SIGMA(F) OF 44.7 IN 1E94 OBSERVED DATA. THEREFORE, ONE SETS OF DYADS IN THE POINT SYMMETRY P622 WERE TWINNING OPERATIONS.

THERE WERE LARGE DISCREPANCIES IN WILSON RATIO (<I^2>/^2) BETWEEN THE OBSERVED DATA AND CALCULATED DATA FROM THE COORDINATE 1E94 IN THE FOLLOWING ZONES: L=2N, L=2N+1, H+K=3N/L=2N; H+K != 3N/L=2N, AND ALL HKL. SOME WERE LARGE THAN 70% AND SOME WERE AS SMALL AS 5%. THIS SUGGESTS THAT X-RAY DATA WERE PROCESSED FROM TWINNED CRYSTALS. THERE WERE ALSO LARGE DIFFERENCES IN THE FIRST AND SECOND MOMENTS OF THE DIFFERENCES (FOBS-FCALC). THIS IS ANOTHER STATISTICAL INDICATOR FOR THE TWINNING PROBLEM.

THE CORRECT POINT GROUP OF 1E94 SHOULD BE LOWER THAN P622, BECAUSE OF LARGE RMERGE VALUES AS INDICATIVE OF NON 50%:50% TWINNING. THE CORRECT SPACE GROUP SHOULD BE EITHER P321 OR P312. THE SPACE GROUP SHOULD ONLY BE DETERMINED FROM THE ORIGINAL INTEGRATED DATA BEFORE SCALING, SO SHOULD BE THE CORRECT TWINNING FRACTION. WITHOUT THE ORIGINAL DATA, THE TWINNING FRACTION COULD ONLY BE TREATED AS 50%:50% AND THE SPACE GROUP SHOULD BE CONSIDERED TO BE EITHER P321 (THIS ENTRY) OR P312 (ENTRY 1HT2). THE DATA TO THE LOWER SPACE GROUP WERE EXPANDED BY THE (-H,-K,L) OPERATION.

THE STRUCTURE FACTOR FILE WAS TAKEN FROM 1E94 AND EXPANDED BY THE (-H,-K,L) OPERATION WITH FREE R-FACTOR SELECTION INCLUDED.

HSLVU, PEPTIDASE-ATPASE COMPLEX, CHAPERONE

SEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEELRHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKV
EATKFTEVGYVGKEVDSIIRDLTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFRK
KLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKIKDAMKLLIEEEAAKLVNPEELKQ
DAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREGVQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPE
LQGRLPIRVELQALTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGARRLHTVLER
LMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL