CRYSTAL STRUCTURE OF PEROXISOMAL DELTA3-DELTA2-ENOYL-COA ISOMERASE FROM SACCHAROMYCES CEREVISIAE

The crystal structure of delta(3)-delta(2)-enoyl-CoA isomerase.

Crystallization and X-ray Diffraction Analysis of Peroxisomal Delta3-Delta2-enoyl-CoA Isomerase from Saccharomyces cerevisiae

MSQEIRQNEKISYRIEGPFFIIHLINPDNLNALEGEDYIYLGELLELADRNRDVYFTIIQSSGRFFSSGADFKGIAKAQG
DDTNKYPSETSKWVSNFVARNVYVTDAFIKHSKVLICCLNGPAIGLSAALVALCDIVYSINDKVYLLYPFANLGLITEGG
TTVSLPLKFGTNTTYECLMFNKPFKYDIMCENGFISKNFNMPSSNAEAFNAKVLEELREKVKGLYLPSCLGMKKLLKSNH
IDAFNKANSVEVNESLKYWVDGEPLKRFRQLGSKQRKHRL

MES, ammonium sulfate, 1,4-dioxane, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K

enoyl-CoA isomerase liganded with perrhenate

MSQEIRQNEKISYRIEGPFFIIHLMNPDNLNALEGEDYIYLGELLELADRNRDVYFTIIQSSGRFFSSGADFKGIAKAQG
DDTNKYPSETSKWVSNFVARNVYVTDAFIKHSKVLICCLNGPAIGLSAALVALCDIVYSINDKVYLLYPFANLGLITEGG
TTVSLPLKFGTNTTYECLMFNKPFKYDIMCENGFISKNFNMPSSNAEAFNAKVLEELREKVKGLYLPSCLGMKKLLKSNH
IDAFNKANSVEVNESLKYWVDGEPLKRFRQLGSKQRKHRL