Synthesis, characterization, and application of cy-dye- and alexa-dye-labeled hongotoxin(1) analogues. The first high affinity fluorescence probes for voltage-gated K+ channels.
Four milligrams of HgTX1 were dissolved in 0.5 ml mixture of H2O and D2O in a 9:1 ratio (v/v). The pH value of the HgTX1 solution was 3.0 uncorrected for isotope effects. Amide proton exchange rate was determined after lyophilization and dissolution in 100% D2O
scorpion, toxin, centruroides limbatus Hgtx1, potassium channel