STRUCTURAL BASIS FOR ALLOSTERIC SUBSTRATE SPECIFICITY REGULATION IN CLASS III RIBONUCLEOTIDE REDUCTASES: NRDD IN COMPLEX WITH DGTP

A Metal-Binding Site in the Catalytic Subunit of Anaerobic Ribonucleotide Reductase.

Structural basis for allosteric substrate specificity regulation in anaerobic ribonucleotide reductases.

SEQUENCE DETERMINATION\: P.YOUNG, M.OHMAN, M.Q.XU.
ANAEROBIC RIBONUCLEOTIDE-TRIPHOSPHATE REDUCTASE

MTIEKEIEGLIHKTNKDLLNENANKDSRVFPTQRDLMAGIVSKHIAKNMVPSFIMKAHESGIIHVHDIDYSPALPFTNCC
LVDLKGMLENGFKLGNAQIETPKSIGVATAIMAQITAQVASHQYGGTTFANVDKVLSPYVKRTYAKHIEDAEKWQIADAL
NYAQSKTEKDVYDAFQAYEYEVNTLFSSNGQTPFVTITFGTGTDWTERMIQKAILKNRIKGLGRDGITPIFPKLVMFVEE
GVNLYKDDPNYDIKQLALECASKRMYPDIISAKNNKAITGSSVPVSPMGCRSFLSVWKDSTGNEILDGRNNLGVVTLNLP
RIALDSYIGTQFNEQKFVELFNERMDLCFEALMCRISSLKGVKATVAPILYQEGAFGVRLKPDDDIIELFKNGRSSVSLG
YIGIHELNILVGRDIGREILTKMNAHLKQWTERTGFAFSLYSTPAENLCYRFCKLDTEKYGSVKDVTDKGWYTNSFHVSV
EENITPFEKISREAPYHFIATGGHISYVELPDMKNNLKGLEAVWDYAAQHLDYFGVNMPVDKCFTCGSTHEMTPTENGFV
CSICGETDPKKMNTIRRTCAYLGNPNERGFNLGKNKEIMHRVKHQ

30% PEG 400, 02M MGCL2, 0.1M HEPES PH 7.5, 7MM DTT

STRUCTURAL BASIS FOR ALLOSTERIC SUBSTRATE SPECIFICITY REGULATION IN CLASS III RIBONUCLEOTIDE REDUCTASES: NRDD IN COMPLEX WITH DCTP.

STRUCTURAL BASIS FOR ALLOSTERIC SUBSTRATE SPECIFICITY REGULATION IN CLASS III RIBONUCLEOTIDE REDUCTASES: NRDD IN COMPLEX WITH DTTP

STRUCTURAL BASIS FOR ALLOSTERIC SUBSTRATE SPECIFICITY REGULATION IN CLASS III RIBONUCLEOTIDE REDUCTASES: NRDD IN COMPLEX WITH DATP.

STRUCTURAL BASIS FOR ALLOSTERIC SUBSTRATE SPECIFICITY REGULATION IN CLASS III RIBONUCLEOTIDE REDUCTASES: NATIVE NRDD

CATALYTIC ACTIVITY INVOLVES 2'-DEOXYRIBONUCLEOSIDE TRIPHOSPHATE
+ OXIDIZED THIOREDOXIN + H(2)O = RIBONUCLEOSIDE TRIPHOSPHATE +
REDUCED THIOREDOXIN.

ENGINEERED MUTATION GLY 580 ALA

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

OXIDOREDUCTASE, REDUCTASE, ALLOSTERIC REGULATION, SUBSTRATE SPECIFICITY