Dihydropyrimidine dehydrogenase (DPD) from pig, ternary complex of a mutant enzyme (C671A), NADPH and 5-fluorouracil

NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

Crystal Structure of Dihydropyrimidine Dehydrogenase, a Major Determinant of the Pharmacokinetics of the Anti-Cancer Drug 5-Fluorouracil

Crystallization and Preliminary X-Ray Study of Pig Liver Dihydropyrimidine Dehydrogenase

NDP A 1032 HAS WRONG CHIRALITY AT ATOM C4D

NDP A 1032 HAS WRONG CHIRALITY AT ATOM C3D

NDP A 1032 HAS WRONG CHIRALITY AT ATOM C2D

NDP B 1032 HAS WRONG CHIRALITY AT ATOM C4D

NDP B 1032 HAS WRONG CHIRALITY AT ATOM C3D

NDP B 1032 HAS WRONG CHIRALITY AT ATOM C2D

NDP C 1032 HAS WRONG CHIRALITY AT ATOM C4D

NDP C 1032 HAS WRONG CHIRALITY AT ATOM C3D

NDP C 1032 HAS WRONG CHIRALITY AT ATOM C2D

NDP D 1032 HAS WRONG CHIRALITY AT ATOM C4D

NDP D 1032 HAS WRONG CHIRALITY AT ATOM C3D

NDP D 1032 HAS WRONG CHIRALITY AT ATOM C2D

DIHYDROURACIL DEHYDROGENASE, DIHYDROTHYMINE DEHYDROGENASE

MAPVLSKDVADIESILALNPRTQSHAALHSTLAKKLDKKHWKRNPDKNCFHCEKLENNFDDIKHTTLGERGALREAMRCL
KCADAPCQKSCPTHLDIKSFITSISNKNYYGAAKMIFSDNPLGLTCGMVCPTSDLCVGGCNLYATEEGSINIGGLQQFAS
EVFKAMNIPQIRNPCLPSQEKMPEAYSAKIALLGAGPASISCASFLARLGYSDITIFEKQEYVGGLSTSEIPQFRLPYDV
VNFEIELMKDLGVKIICGKSLSENEITLNTLKEEGYKAAFIGIGLPEPKTDDIFQGLTQDQGFYTSKDFLPLVAKSSKAG
MCACHSPLPSIRGAVIVLGAGDTAFDCATSALRCGARRVFLVFRKGFVNIRAVPEEVELAKEEKCEFLPFLSPRKVIVKG
GRIVAVQFVRTEQDETGKWNEDEDQIVHLKADVVISAFGSVLRDPKVKEALSPIKFNRWDLPEVDPETMQTSEPWVFAGG
DIVGMANTTVESVNDGKQASWYIHKYIQAQYGASVSAKPELPLFYTPVDLVDISVEMAGLKFINPFGLASAAPTTSSSMI
RRAFEAGWGFALTKTFSLDKDIVTNVSPRIVRGTTSGPMYGPGQSSFLNIELISEKTAAYWCQSVTELKADFPDNIVIAS
IMCSYNKNDWMELSRKAEASGADALELNLSAPHGMGERGMGLACGQDPELVRNICRWVRQAVQIPFFAKLTPNVTDIVSI
ARAAKEGGADGVTATNTVSGLMGLKADGTPWPAVGAGKRTTYGGVSGTAIRPIALRAVTTIARALPGFPILATGGIDSAE
SGLQFLHSGASVLQVCSAVQNQDFTVIQDYCTGLKALLYLKSIEELQGWDGQSPGTESHQKGKPVPRIAELMGKKLPNFG
PYLEQRKKIIAEEKMRLKEQNAAFPPLERKPFIPKKPIPAIKDVIGKALQYLGTFGELSNIEQVVAVIDEEMCINCGKCY
MTCNDSGYQAIQFDPETHLPTVTDTCTGCTLCLSVCPIIDCIRMVSRTTPYEPKRGLPLAVNPVC

100 MM SODIUM CITRATE PH 4.7, 16-20 % POLYETHYLENE GLYCOL 6000, 1 MM DTT, 1 MM NADPH, 5 MM 5FU

DIHYDROPYRIMIDINE DEHYDROGENASE (DPD) FROM PIG

SHEET
DETERMINATION METHOD: DSSP
THE SHEETS PRESENTED AS "AF, BF, CF, DF" IN EACH CHAIN ON
SHEET RECORDS ARE ACTUALLY AN 8-STRANDED BARREL THIS IS
REPRESENTED BY A 9-STRANDED SHEET IN WHICH THE FIRST AND
LAST STRANDS ARE IDENTICAL.

CHAIN A, B, C, D ENGINEERED MUTATION CYS671ALA INVOLVED IN THE CATABOLISM OF URACIL AND THYMIDINE LEADING TO THE FORMATION OF BETA-ALANINE. CATALYSES THE REDUCTION OF URACIL AND THYMINE.

DIHYDROPYRIMIDINE DEHYDROGENASE, UNCOMPLEXED

THE C-TERMINAL RESIDUES WERE NOT SEEN IN THE DENSITY
ELECTRON TRANSFER, FLAVIN, IRON-SULFUR CLUSTERS, PYRIMIDINE CATABOLISM, 5-FLUOROURACIL DEGRADATION, OXIDOREDUCTASE