CRYSTAL STRUCTURE OF CATALASE HPII FROM ESCHERICHIA COLI, ASN201ALA VARIANT.

CIS-HEME D HYDROXYCHLORIN GAMMA-SPIROLACTONE

Substrate flow in catalases deduced from the crystal structures of active site variants of HPII from Escherichia coli.

Crystal Structure of Catalase HPII from Escherichia coli

Structure of the Heme D of Penicillium Vitale and Escherichia Coli Catalases

Structure of Catalase HpII from Escherichia Coli at 1.9 A Resolution

MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTPPGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYAL
TTNQGVRIADDQNSLRAGSRGPTLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGANTPIFFIQDAHKFPDFVHAVKPEPHWAIPQGQSAHDTFW
DYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHTFRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRR
ELWEAIEAGDFPEYELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFHPGHIVPGL
DFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHRMGIDTNPANYEPNSINDNWPRETPPGPKRG
GFESYQERVEGNKVRERSPSFGEYYSHPRLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAK
NLGIELTDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALKAKGVHAKLLYSRMGEV
TADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDANYYLMEAYKHLKPIALAGDARKFKATIKIADQGEEGIVE
ADSADGSFMDELLTLMAAHRVWSRIPKIDKIPA

PEG 3350, LiCl, Tris-HCl, pH 9.0, vapor diffusion/hanging drop, temperature 297.0K

1IPH contains the structure of native protein at 2.8 A resolution.

1CF9 contains the structure of Val169Cys variant at 1.8 A resolution.

1Qf7 contains the structure of His992Gln variant at 1.9 A resolution.

1GG9 contains the structure of His128Asn variant at 1.9 A resolution.

1GGE contains the structure of native protein with cis-heme d hydroxychlorin gamma-spirolactone at 1.9 A resolution.

1GGF contains the structure of His128Asn variant complex with hydrogen peroxide at 2.28 A resolution.

1GGH contains the structure of His128Ala variant at 2.15 A resolution.

1GGK contains the structure of Asn201His variant at 2.26 A resolution.

REFMAC, WEIGHT MATRIX 0.2. X-plor was also used for refinement.
BETA BARREL, ALPHA HELICAL DOMAIN, FLAVODOXIN LIKE DOMAIN, OXIDOREDUCTASE

MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTPPGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYAL
TTNQGVRIADDQNSLRAGSRGPTLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFVHAVKPEPHWAIPQGQSAHDTFW
DYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHTFRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRR
ELWEAIEAGDFPEYELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFHPGHIVPGL
DFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHRMGIDTNPANYEPNSINDNWPRETPPGPKRG
GFESYQERVEGNKVRERSPSFGEYYSHPRLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAK
NLGIELTDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALKAKGVHAKLLYSRMGEV
TADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDANYYLMEAYKHLKPIALAGDARKFKATIKIADQGEEGIVE
ADSADGSFMDELLTLMAAHRVWSRIPKIDKIPA