TERNARY COMPLEX BETWEEN PSYCHROPHILIC ALPHA-AMYLASE, COMII (PSEUDO TRI-SACCHARIDE FROM BAYER) AND TRIS (2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL)

4,6-dideoxy-4-{[(1S,5R,6S)-3-formyl-5,6-dihydroxy-4-oxocyclohex-2-en-1-yl]amino}-alpha-D-xylo-hex-5-enose; 4,6-dideoxy-4-{[(1S,5R,6S)-3-formyl-5,6-dihydroxy-4-oxocyclohex-2-en-1-yl]amino}-D-xylo-hex-5-enose; 4,6-dideoxy-4-{[(1S,5R,6S)-3-formyl-5,6-dihydroxy-4-oxocyclohex-2-en-1-yl]amino}-xylo-hex-5-enose

Crystallographic evidence of a transglycosylation reaction: ternary complexes of a psychrophilic alpha-amylase.

STRUCTURES OF THE PSYCHROPHILIC ALTEROMONAS HALOPLANCTIS ALPHA-AMYLASE GIVE INSIGHTS INTO COLD ADAPTATION AT A MOLECULAR LEVEL

CRYSTAL STRUCTURES OF THE PSYCHROPHILIC ALPHA-AMYLASE FROM ALTEROMONAS HALOPLANCTIS IN ITS NATIVE FORM AND COMPLEXED WITH AN INHIBITOR

CRYSTALLIZATION AND PRELIMINARY X-RAY DIFFRACTION STUDIES OF ALPHA-AMYLASE FROM THE ANTARCTIC PSYCHROPHILE ALTEROMONAS HALOPLANCTIS A23

TPTTFVHLFEWNWQDVAQECEQYLGPKGYAAVQVSPPNEHITGSQWWTRYQPVSYELQSRGGNRAQFIDMVNRCSAAGVD
IYVDTLINHMAAGSGTGTAGNSFGNKSFPIYSPQDFHESCTINNSDYGNDRYRVQNCELVGLADLDTASNYVQNTIAAYI
NDLQAIGVKGFRFDASKHVAASDIQSLMAKVNGSPVVFQEVIDQGGEAVGASEYLSTGLVTEFKYSTELGNTFRNGSLAW
LSNFGEGWGFMPSSSAVVFVDNHDNQRGHGGAGNVITFEDGRLYDLANVFMLAYPYGYPKVMSSYDFHGDTDAGGPNVPV
HNNGNLECFASNWKCEHRWSYIAGGVDFRNNTADNWAVTNWWDNTNNQISFGRGSSGHMAINKEDSTLTATVQTDMASGQ
YCNVLKGELSADAKSCSGEVITVNSDGTINLNIGAWDAMAIHKNAKLN

MPD, Hepes, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K

THE SAME PROTEIN (ALPHA-AMYLASE FROM PSEUDOALTEROMONAS HALOPLANCTIS) IN ITS NATIVE STATE

THE SAME PROTEIN (ALPHA-AMYLASE FROM PSEUDOALTEROMONAS HALOPLANCTIS) IN COMPLEX WITH TRIS

THE SAME PROTEIN (PSYCHROPHILIC ALPHA-AMYLASE EXPRESSED IN E.COLI) IN ITS NATIVE STATE

THE SAME PROTEIN (ALPHA-AMYLASE FROM PSEUDOALTEROMONAS HALOPLANCTIS) IN A TERNARY COMPLEX WITH ACARBOSE (PSEUDO-TETRA SACCHARIDE FROM BAYER) AND TRIS

HETEROGEN
HET GROUP USED IN THE FILE AS DAF IS ALSO KNOWN AS:
ACARVIOSINE (VALIENAMINE + 4-AMINO-4, 6-DIDEOXY-A-D-GLUCOSE)

Following residues have been refined in double conformations: Arg 49, Thr 96, Ser 102, Arg 234, Arg 282, Arg 373, Val 420

ALPHA-BETA 8 BARREL, 3 DOMAIN STRUCTURE, HYDROLASE