SOLUTION STRUCTURE AND RELAXATION MEASUREMENTS OF AN ANTIGEN-FREE HEAVY CHAIN VARIABLE DOMAIN (VHH) FROM LLAMA
Solution structure and backbone dynamics of an antigen-free heavy chain variable domain (VHH) from Llama
QVQLQESGGGLVQAGGSLRLSCAASGRTGSTYDMGWFRQAPGKERESVAAINWDSARTYYASSVRGRFTISRDNAKKTVY LQMNSLKPEDTAVYTCGAGEGGTWDSWGQGTQVTVSS
Structures with acceptable covalent geometry, structures with favorable non-bond energy, structures with the least restraint violations,structures with the lowest energy
The structures are based on a total of 1295 restraints, 1181 are NOE-derived distance constraints, 57 dihedral angle restraints, 51 distance restraints from hydrogen bonds and 6 from the disulfide bridge.
3mM 15N H14; 10mM deuterated Na acetate pH4.6