STRUCTURE OF RECOMBINANT HUMAN UBIQUITIN IN AOT REVERSE MICELLES
Validation of protein structure from preparations of encapsulated proteins dissolved in low viscosity fluids.
High-resolution NMR of encapsulated proteins dissolved in low-viscosity fluids
Preparation of encapsulated proteins dissolved in low viscosity fluids
structure of ubiquitin in aqueous buffer
The structures are based on 1805 NOEs and 63 phi restraints
4 mg c13/n15 ubiquitin in 13.5 ul of buffer (50mM Sodium acetate, 250mM Sodium Chloride, pH 5.0) and 1 ml D12-pentane
75mM bis(2-ethyl hexyl) sulfosuccinate in d-pentane
8 mg 13/n15 ubiquitin in 27 ul of buffer (50mM Sodium acetate, 250mM Sodium Chloride, pH 5.0)
75mM bis(2-ethyl hexyl) sulfosuccinate in butane
4 mg n15 ubiquitin in 13.5 ul of buffer (50mM Sodium acetate, 250mM Sodium Chloride, pH 5.0)
Guntert, P., Mumenthaler, C., Wuthrich, K.
Bartels, C.X., Guntert, P., Billeter, M., Wuthrich, K.
reverse micelle, AOT, encapsulation, gene regulation, cell cycle