NMR solution structure and backbone dynamics of the CC chemokine eotaxin-3.
SMALL INDUCIBLE CYTOKINE A26, MACROPHAGE INFLAMMATORY PROTEIN 4-ALPHA
Solution Structure of Eotaxin-3, Ensemble of 20 structures
The structures are based on a total of 1250 restraints, 1157 are NOE-derived distance constraints, 69 dihedral angle restraints, 17 distance restraints from hydrogen bonds.
distance geometry-simulated annealing protocol implemented in XPLOR
2 mM eotaxin-3,U-15N, 20mM sodium acetate (deteurated), 90% H2O, 10% D2O
2 mM eotaxin-3,U-15N, 13C, 20mM sodium acetate (deteurated), 90% H2O, 10% D2O
2 mM eotaxin-3,U-15N, 13C, 20mM sodium acetate (deteurated), 100% D2O
0.5 mM eotaxin-3,10% 13C-labeled, 20mM sodium acetate (deteurated), 100% D2O
MMGLSLASAVLLASLLSLHLGTATRGSDISKTCCFQYSHKPLPWTWVRSYEFTSNSCSQRAVIFTTKRGKKVCTHPRKKW VQKYISLLKTPKQL