MINIMAL CONFORMATION OF THE ALPHA-CONOTOXIN IMI FOR THE ALPHA7 NEURONAL NICOTINIC ACETYLCHOLINE RECEPTOR RECOGNITION
Minimal conformation of the alpha-conotoxin ImI for the alpha7 neuronal nicotinic acetylcholine receptor recognition: correlated CD, NMR and binding studies.
A Nicotinic Acetylcholine Receptor Ligand of Unique Specificity, alpha-Conotoxin ImI
Functional Determinants by which Snake and Cone Snail Toxins Block the alpha 7 Neuronal Nicotinic Acetylcholine Receptors
Identification of Residues in the Neuronal alpha7 Acetylcholine Receptor that Confer Selectivity for Conotoxin ImI
NMR Spatial Structure of alpha-Conotoxin ImI Reveals a Common Scaffold in Snail and Snake Toxins Recognizing Neuronal Nicotinic Acetylcholine Receptors
NMR Solution Structure of alpha-Conotoxin ImI and Comparison to other Conotoxins Specific for Neuronal Nicotinic Acetylcholine Receptors
The solid phase synthesis of alpha-conotoxin ImI was carried out using the F-moc chemistry. The sequence of the protein is naturally found in the venom of Conus imperialis (Imperial cone).
This structure was determined using standard 2D homonuclear techniques.
structures with the least restraint violations,structures with the lowest energy
The structures were generated using the force fields parallhdg.pro and CHARMM22 of X-PLOR.
5-6 mM alpha-ImI; 90 % H2O, 10 % D2O; 0.1 M potassium phosphate buffer pH 5.8
90 % H2O, 10 % D2O; 0.1 M potassium phosphate buffer pH 5.8