TOLUENE-4-MONOOXYGENASE CATALYTIC EFFECTOR PROTEIN NMR STRUCTURE
Solution structure of the toluene 4-monooxygenase effector protein (T4moD).
Assignment of 1H, 13C and 15N NMR Signals in the Toluene 4-monooxygenase Effector Protein
Application of Fed-batch Fermentation to the Preparation of Isotopicaly Labeled or Selenomethionyl-labeled Proteins
Detection and Classification of Hyperfine-shifted 1H, 2H, and 15N Resonances of the Rieske Ferredoxin Component from Toluene 4-monooxygenase
Recombinant Toluene-4-monooxygenase: Catalytic and Mossbauer Studies of the Purified Diiron and Rieske Components of a Four-Protein Complex
Toluene-4-monooxygenase, a Three Component Enzyme System that Catalyzes the Oxidation of Toluene to p-Cresol in Pseudomonas mendocina KR1
TOLUENE-4-MONOOXYGENASE CATALYTIC EFFECTOR
TOLUENE-4-MONOOXYGENASE SYSTEM PROTEIN D, T4MOD
STLADQALHNNNVGPIIRAGDLVEPVIETAEIDNPGKEITVEDRRAYVRIAAEGELILTRKTLEEQLGRPFNMQELEINL ASFAGQIQADEDQIRFYFDKTM
123 1H-15N CROSS PEAKS WERE ASSIGNED OUT OF THE EXPECTED 129 CROSS PEAKS (95% COMPLETE). 386 BACKBONE RESONANCES WERE ASSIGNED OUT OF THE EXPECTED 400 RESONANCES (97% COMPLETE). 789 SIDECHAIN RESONANCES WERE ASSIGNED OUT OF THE EXPECTED 901 RESONANCES (88% COMPLETE). ARG SIDECHAIN ATOMS ACCOUNT FOR 51 OF 112 UNASSIGNED SIDECHAIN ATOMS (51%).
1H-15N HSQC,HNCA,HN(CO)CA,C(CO)NH,HNCACB,HCCH-COSY,HCCH-TOCSY,2D_1H-NOESY,2D_1H-TOCSY,CT-13C-HSQC,3D_NOESY-CT-HSCQ,3D_TOCSY-CT-HSQC
THE STRUCTURES ARE BASED ON 1339 interproton restraints. These comprised 367 intraresidue, 424 sequential, 195 medium-range, and 273 long-range NOE distance restraints and 128 dihedral angle restraints (78 psi, 26 phi, and 24 chi), AND 80 HYDROGEN BONDING reSTRAINTS.
DISTANCE GEOMETRY SIMULATED ANNEALING, TORSION ANGLE MOLECULAR DYNAMICS
50 mM phosphate pH 7.0, 90% H20/10% D20 or 99.9% D20, 0.5 uM azide, protease inhibitor cocktail product no. p 27124, Sigma Chemical Co, St. Louis, MO
aromatic hydrocarbon catabolism, oxidoreductase, monooxygenase, toluene oxidation