SERENDIPITOUS CRYSTAL STRUCTURE CONTAINING THE HEAT SHOCK TRANSCRIPTION FACTOR'S DNA BINDING DOMAIN AND COGNATE DNA THAT IS TRANSLATIONALLY DISORDERED
Crystal packing interaction that blocks crystallization of a site-specific DNA binding protein-DNA complex.
A new use for the 'wing' of the 'winged' helix-turn-helix motif in the HSF-DNA cocrystal
ARPAFVNKLWS(MSE)VNDKSNEKFIHWSTSGESIVVPNRERFVQEVLPKYFKHSNFASFVRQLN(MSE)YGWHKVQDVK SGS(MSE)LSNNDSRWEFENERHA
ARPAFVNKLWSMVNDKSNEKFIHWSTSGESIVVPNRERFVQEVLPKYFKHSNFASFVRQLNMYGWHKVQDVKSGSMLSNN DSRWEFENERHA
PEG 4000, Cacodylate, Ammonium Acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
3HTS is the HEAT SHOCK TRANSCRIPTION FACTOR DNA-BINDING DOMAIN/DNA COMPLEX
2HTS is the heat shock transcription factor DNA-binding domain
1FYL is the serendipitous crystal structure containing the heat shock transcription factor's DNA binding domain and cognate DNA in a head-to-head orientation
1FYM is the serendipitous crystal structure containing the heat shock transcription factor's DNA binding domain and cognate DNA in a tail-to-tail orientation
This sequence is based on an idealized HSE sequence.
The structure was originally refined by mistake with methionine in lieu of selenomethionine. The deposited coordinates have been adjusted to contain selenomethionine. Because the refinement used group B-factors, the B-factors were kept the same.
protein and DNA are not interacting in a physiologically relevant manner
crystal-packing interface, crystallization, protein-DNA interface, protein-protein interface, static disorder, TRANSCRIPTION-DNA COMPLEX
ARPAFVNKLWSMVNDKSNEKFIHWSTSGESIVVPNRERFVQEVLPKYFKHSNFASFVRQLNMYGWHKVQDVKSGSMLSNN DSRWEFENENFK