STRUCTURAL ANALYSIS OF ADENYLATE CYCLASES FROM TRYPANOSOMA BRUCEI IN THEIR MONOMERIC STATE

RECEPTOR-TYPE ADENYLATE CYCLASE GRESAG 4.1

NNNRAPKEPTDPVTLIFTDIESSTALWAAHPDLMPDAVAAHHRMVRSLIGRYKCYEVKTVGDSFMIASKSPFAAVQLAQE
LQLCFLHHDWGTNALDDSYREFEEQRAEGECEYTPPTAHMDPEVYSRLWNGLRVRVGIHTGLCDIRHDEVTKGYDYYGRT
PNMAARTESVANGGQVLMTHAAYMSLSAEDRKQIDVTALGDVALRGVSDPVKMYQLNTVPSRNFAALRLDREYFD

20 mM Tris/HCl, 1.8-1.9 M ammonium sulfate, pH 6.5, VAPOR DIFFUSION, temperature 18K

1FX4 is the structure of the catalytic domain of GRESAG4.3, an isozyme of GRESAG4.1

The biological assembly in the catalytic state is a dimer

cAMP, trypanosomes, adenylyl cyclases, monomer-dimer, catalysis, LYASE

NNNRAPKEPTDPVTLIFTDIESSTALWAAHPDLMPDAVAAHHRMVRSLIGRYKCYEVKTVGDSFMIASKSPFAAVQLAQE
LQLCFLHHDWGTNALDDSYREFEEQRAEGECEYTPPTAHMDPEVYSRLWNGLRVRVGIHTGLCDIIRHDEVTKGYDYYGR
TPNMAARTESVANGGQVLMTHAAYMSLSAEDRKQIDVTALGDVALRGVSDPVKMYQLNTVPSRNFAALRLDREYFD