Crystal Structure of Fasciculin 2 from Green Mamba Snake Venom: Evidence for Unusual Loop Flexibility
Structure of fasciculin 2 from green mamba snake venom: evidence for unusual loop flexibility.
1.9-Angstroms Resolution Structure of Fasciculin 1, an Anti-Acetylcholinesterase Toxin from Green Mamba Snake Venom
Crystals of Fasciculin 2 from Green Mamba Snake Venom
SHEET SHEET SHEET_ID: AB, DETERMINATION METHOD: DSSP.
THE CRYSTALS ONLY GROW IN THE PRESENCE OF BETA-OCTYL GLUCOSIDE. DURING REFINEMENT THE FO-FC ELECTRON DENSITY MAP SHOWED A LARGE POSITIVE PEAK IN A HYDROPHOBIC POCKET AT THE INTERFACE BETWEEN THE TWO MOLECULES FORMING THE CRYSTALLOGRAPHIC DIMER. FOR THE PURPOSE OF REFINEMENT, TWO OXYGEN ATOMS WERE MODELED IN THIS DENSITY. VAN DER WAALS INTERACTIONS BETWEEN THESE TWO ATOMS WERE INTENTIONALLY SWITCHED OFF TO LET THEM FREE TO MOVE TO THE OPTIMAL POSITION. IT EXPLAINS THE SHORT DISTANCE (1.7 A) BETWEEN THEM. THE AUTHORS DISCUSS THIS IN THE PAPER CITED ON JRNL RECORDS AND BELIEVE THAT THIS POCKET COULD BE OCCUPIED BY PART OF THE ALIPHATIC CHAIN OF THE BETA-OCTYL GLUCOSIDE MOLECULE. THE TWO OXYGENS ARE PRESENTED AS O 156 AND O 157 BELOW.
FASCICULIN 2 (SYNCHROTRON X-RAY DIFFRACTION)