NMR STRUCTURE OF THE C TERMINAL DOMAIN OF CARDIAC TROPONIN C BOUND TO THE N TERMINAL DOMAIN OF CARDIAC TROPONIN I.
Solution structures of the C-terminal domain of cardiac troponin C free and bound to the N-terminal domain of cardiac troponin I.
MVRCMKDDSKGKTEEELSDLFRMFDKNADGYIDLEELKIMLQATGETITEDDIEELMKDGDKNNDGRIDYDEFLEFMKGV E
C TERMINAL DOMAIN OF CARDIAC TROPONIN C (81- 161) BOUND TO THE N TERMINAL DOMAIN OF CARDIAC TROPONIN I (33-80), CALCIUM IONS BOUND AT SITES III AND IV
BEST 20 STRUCTURES. THESE STRUCTURES WERE DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON CA2+_SATURATED 15N, 13C-CTNC(81-161) BOUND TO CTNI(33-80).
100mM KCL,15mM CACL2, 100mM KCL,15mM CACL2
REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE
1mM [15N,13C]cTnC(81-161)/cTnI(33-80); 20mM Tris-d11; 100mM KCl; 20mM DTT; 15mM CaCl2; 0.1mM Leupetin; 0.1mM pefabloc; 90% H2O/10% D2O
TROPONIN C-TROPONIN I INTERACTION, CARDIAC, MUSCLE PROTEIN, CALCIUM BINDING PROTEIN, CONTRACTILE PROTEIN