SOLUTION STRUCTURE OF HCV IRES RNA DOMAIN IIIE
Structures of two RNA domains essential for hepatitis C virus internal ribosome entry site function.
HCV IRES RNA DOMAIN IIIE (NUCLEOTIDES 290-303 OF GENOMIC HEPATITIS C VIRAL RNA)
Synthesized enzymatically in-vitro using T7 RNA Polymerase
THE STRUCTURE WAS DETERMINED USING A NON-ISOTOPICALLY LABELLED RNA SAMPLE. NATURAL ABUNDANCE 13C HMQC EXPERIMENTS WERE PERFORMED.
structures with the least restraint violations,structures with the lowest energy
31P-decoupled DQF COSY, HP COSY, D2O NOESY, H2O NOESY
Simulated annealing and restrained molecular dynamics
sodium phosphate buffer, 96% H2O/4% D2O or 100% D2O
Ribonucleic Acid, Hepatitis C virus internal ribosome entry site, Hairpin loop, Tetraloop, RNA structure, RNA