CRYSTAL STRUCTURE ANALYSIS OF N-ACETYLNEURAMINATE LYASE FROM HAEMOPHILUS INFLUENZAE: CRYSTAL FORM I

Active site modulation in the N-acetylneuraminate lyase sub-family as revealed by the structure of the inhibitor-complexed Haemophilus influenzae enzyme.

MRDLKGIFSALLVSFNEDGTINEKGLRQIIRHNIDKMKVDGLYVGGSTGENFMLSTEEKKEIFRIAKDEAKDQIALIAQV
GSVNLKEAVELGKYATELGYDCLSAVTPFYYKFSFPEIKHYYDTIIAETGSNMIVYSIPFLTGVNMGIEQFGELYKNPKV
LGVKFTAGDFYLLERLKKAYPNHLIWAGFDEMMLPAASLGVDGAIGSTFNVNGVRARQIFELTKAGKLKEALEIQHVTND
LIEGILANGLYLTIKELLKLEGVDAGYCREPMTSKATAEQVAKAKDLKAKFLS

Tris-HCl, PEG 4000, NaOAc, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 20.0K

N-acetylneuraminate lyase from Escherichia coli Izard, T., Lawrence, M.C., Malby, R.L., Lilley, G.G., Colman, P.M.: The three-dimensional struture of N-acetylneuraminate lyase Escherichia coli. Structure (1994) 2, 361.

Structure and Mechansism of a Sub-Family of Enzymes Related to N-acetylneuraminate Lyase
M.C. Lawrence, J.A.R.G. Barbosa, B.J. Smith, N.E. Hall, P.A. Pilling, H.C. Ooi, S.M. Marcuccio
J. Mol. Biol. (1997) 266, 381-399

Structure and Mechanism of a Sub-Family of Enzymes Related to N-acetylneuraminate Lyase
M.C. Lawrence, J.A.R.G. Barbosa, B.J. Smith, N.E. Hall, P.A. Pilling, H.C. Ooi, S.M. Marcuccio
J. Mol. Biol. (1997) 266, 381-399

The biological assembly is a tetramer from chains A, B, C and D.

MRDLKGIFSALLVSFNEDGTINEKGLRQIIRHNIDKMKVDGLYVGGSTGENFMLSTEEKKEIFRIAKDEAKDQIALIAQV
GSVNLKEAVELGKYATELGYDCLSAVTPFYYKFSFPEIKHYYDTIIAETGNNMIVYSIPFLTGVNMGIEQFGELYKNPKV
LGVKFTAGDFYLLERLKKAYPNHLIWAGFDEMMLPAASLGVDGAIGSTFNVNGVRARQIFELTKAGKLAEALEIQHVTND
LIEGILANGLYLTIKELLKLEGVDAGYCREPMTSKATEEQLAKAKDLKAKFLS