SOLUTION STRUCTURE OF THE N-TERMINAL DOMAIN OF THE TNFR1 ASSOCIATED PROTEIN, TRADD.
Solution structure of N-TRADD and characterization of the interaction of N-TRADD and C-TRAF2, a key step in the TNFR1 signaling pathway.
TUMOR NECROSIS FACTOR RECEPTOR TYPE 1 ASSOCIATED DEATH DOMAIN PROTEIN
TRADD, TNFR1-ASSOCIATED DEATH DOMAIN PROTEIN
MAAGQNGHEEWVGSAYLFVESSLDKVVLSDAYAHPQQKVAVYRALQAALAESGGSPDVLQMLKIHRSDPQLIVQLRFCGR QPCGRFLRAYREGALRAALQRSLAAALAQHSVPLQLELRAGAERLDALLADEERCLSCILAQQPDRLRDEELAELEDALR NLKCGSGAR
Other experiments used were HC(CO)NH_TOCSY, 3D 13C-13C long range correlation, methyl-methyl NOE
Low energies and violations not larger than 0.3A for NOEs and 5 degrees for torsional restraints
Structures determined from 2402 restraints (1883 NOE restraints, 159 dihedral, 100 distance restraints from hydrogen bonds and 240 Alpha and Beta 13C chemical shifts).
20mM Imidazole pH 6.6 200 mMNaCL 20 mM DTT 0.05%NaN3 90%H2O 10% D2O
20mM Imidazole pH 6.6 200 mMNaCL 220 mM DTT 0.05%NaN3 100% D2O