SOLUTION STRUCTURE OF THE CYSTEINE-RICH DOMAIN OF THE ESCHERICHIA COLI CHAPERONE PROTEIN DNAJ.
SYNTHETIC GENE BASED ON E. COLI DNAJ CRD SEQUENCE
The sequence-specific assignments were completed using standard double and triple resonance NMR experiments.
structures with the least restraint violations,structures with the lowest energy
The structures are based on 1361 unique NOE-based distance restraints and 99 torsion angle restaints and 24 stereo assignments.
torsion angle dynamics followed by simulated annealing
600 uM DnaJ CRD U-15N,13C; 20 mM Tris-d11 pH 6.8; 300 uM ZnCl2; 50 mM NaCl; 2 mM DTT; 0.02% NaN3
600 uM DnaJ CRD U-15N; 20 mM Tris-d11 pH 6.8; 300 uM ZnCl2; 50 mM NaCl;
extended beta-hairpin, CXXCXGXG, zinc-binding motif, CHAPERONE